Expression of the GAF Sensor, Carbohydrate-Active Enzymes, Elicitins, and RXLRs Differs Markedly Between Two Phytophthora cactorum Isolates.
Identifieur interne : 000513 ( Main/Exploration ); précédent : 000512; suivant : 000514Expression of the GAF Sensor, Carbohydrate-Active Enzymes, Elicitins, and RXLRs Differs Markedly Between Two Phytophthora cactorum Isolates.
Auteurs : Anna Toljamo [Finlande] ; Daniel Blande [Finlande] ; Mustafa Munawar [Finlande] ; Sirpa O. K Renlampi [Finlande] ; Harri Kokko [Finlande]Source :
- Phytopathology [ 0031-949X ] ; 2019.
Descripteurs français
- KwdFr :
- MESH :
- enzymologie : Phytophthora.
- microbiologie : Fragaria, Maladies des plantes.
- pathogénicité : Phytophthora.
- Glucides, Métabolisme lipidique, Métabolisme secondaire, Phytophthora, Transcriptome, Virulence.
English descriptors
- KwdEn :
- MESH :
- chemical : Carbohydrates.
- classification : Phytophthora.
- enzymology : Phytophthora.
- microbiology : Fragaria, Plant Diseases.
- pathogenicity : Phytophthora.
- Lipid Metabolism, Secondary Metabolism, Transcriptome, Virulence.
Abstract
The phytopathogen Phytophthora cactorum infects economically important herbaceous and woody plant species. P. cactorum isolates differ in host specificity; for example, strawberry crown rot is often caused by a specialized pathotype. Here we compared the transcriptomes of two P. cactorum isolates that differ in their virulence to garden strawberry (Pc407: high virulence; Pc440: low virulence). De novo transcriptome assembly and clustering of contigs resulted in 19,372 gene clusters. Two days after inoculation of Fragaria vesca roots, 3,995 genes were differently expressed between the P. cactorum isolates. One of the genes that were highly expressed only in Pc407 encodes a GAF sensor protein potentially involved in membrane trafficking processes. Two days after inoculation, elicitins were highly expressed in Pc407 and lipid catabolism appeared to be more active than in Pc440. Of the carbohydrate-active enzymes, those that degrade pectin were often more highly expressed in Pc440, whereas members of glycosyl hydrolase family 1, potentially involved in the metabolism of glycosylated secondary metabolites, were more highly expressed in Pc407 at the time point studied. Differences were also observed among the RXLR effectors: Pc407 appears to rely on a smaller set of key RXLR effectors, whereas Pc440 expresses a greater number of RXLRs. This study is the first step toward improving understanding of the molecular basis of differences in the virulence of P. cactorum isolates. Identification of the key effectors is important, as it enables effector-assisted breeding strategies toward crown rot-resistant strawberry cultivars.
DOI: 10.1094/PHYTO-04-18-0136-R
PubMed: 30412010
Affiliations:
Links toward previous steps (curation, corpus...)
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K Renlampi</name><affiliation wicri:level="1"><nlm:affiliation>Department of Environmental and Biological Sciences, University of Eastern Finland, FI-70211 Kuopio, Finland.</nlm:affiliation><country xml:lang="fr">Finlande</country><wicri:regionArea>Department of Environmental and Biological Sciences, University of Eastern Finland, FI-70211 Kuopio</wicri:regionArea><wicri:noRegion>FI-70211 Kuopio</wicri:noRegion></affiliation></author><author><name sortKey="Kokko, Harri" sort="Kokko, Harri" uniqKey="Kokko H" first="Harri" last="Kokko">Harri Kokko</name><affiliation wicri:level="1"><nlm:affiliation>Department of Environmental and Biological Sciences, University of Eastern Finland, FI-70211 Kuopio, Finland.</nlm:affiliation><country xml:lang="fr">Finlande</country><wicri:regionArea>Department of Environmental and Biological Sciences, University of Eastern Finland, FI-70211 Kuopio</wicri:regionArea><wicri:noRegion>FI-70211 Kuopio</wicri:noRegion></affiliation></author></analytic><series><title level="j">Phytopathology</title><idno type="ISSN">0031-949X</idno><imprint><date when="2019" type="published">2019</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Carbohydrates (MeSH)</term><term>Fragaria (microbiology)</term><term>Lipid Metabolism (MeSH)</term><term>Phytophthora (classification)</term><term>Phytophthora (enzymology)</term><term>Phytophthora (pathogenicity)</term><term>Plant Diseases (microbiology)</term><term>Secondary Metabolism (MeSH)</term><term>Transcriptome (MeSH)</term><term>Virulence (MeSH)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Fragaria (microbiologie)</term><term>Glucides (MeSH)</term><term>Maladies des plantes (microbiologie)</term><term>Métabolisme lipidique (MeSH)</term><term>Métabolisme secondaire (MeSH)</term><term>Phytophthora (classification)</term><term>Phytophthora (enzymologie)</term><term>Phytophthora (pathogénicité)</term><term>Transcriptome (MeSH)</term><term>Virulence (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" xml:lang="en"><term>Carbohydrates</term></keywords><keywords scheme="MESH" qualifier="classification" xml:lang="en"><term>Phytophthora</term></keywords><keywords scheme="MESH" qualifier="enzymologie" xml:lang="fr"><term>Phytophthora</term></keywords><keywords scheme="MESH" qualifier="enzymology" xml:lang="en"><term>Phytophthora</term></keywords><keywords scheme="MESH" qualifier="microbiologie" xml:lang="fr"><term>Fragaria</term><term>Maladies des plantes</term></keywords><keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Fragaria</term><term>Plant Diseases</term></keywords><keywords scheme="MESH" qualifier="pathogenicity" xml:lang="en"><term>Phytophthora</term></keywords><keywords scheme="MESH" qualifier="pathogénicité" xml:lang="fr"><term>Phytophthora</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Lipid Metabolism</term><term>Secondary Metabolism</term><term>Transcriptome</term><term>Virulence</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Glucides</term><term>Métabolisme lipidique</term><term>Métabolisme secondaire</term><term>Phytophthora</term><term>Transcriptome</term><term>Virulence</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The phytopathogen <i>Phytophthora cactorum</i> infects economically important herbaceous and woody plant species. <i>P. cactorum</i> isolates differ in host specificity; for example, strawberry crown rot is often caused by a specialized pathotype. Here we compared the transcriptomes of two <i>P. cactorum</i> isolates that differ in their virulence to garden strawberry (Pc407: high virulence; Pc440: low virulence). De novo transcriptome assembly and clustering of contigs resulted in 19,372 gene clusters. Two days after inoculation of <i>Fragaria vesca</i> roots, 3,995 genes were differently expressed between the <i>P. cactorum</i> isolates. One of the genes that were highly expressed only in Pc407 encodes a GAF sensor protein potentially involved in membrane trafficking processes. Two days after inoculation, elicitins were highly expressed in Pc407 and lipid catabolism appeared to be more active than in Pc440. Of the carbohydrate-active enzymes, those that degrade pectin were often more highly expressed in Pc440, whereas members of glycosyl hydrolase family 1, potentially involved in the metabolism of glycosylated secondary metabolites, were more highly expressed in Pc407 at the time point studied. Differences were also observed among the RXLR effectors: Pc407 appears to rely on a smaller set of key RXLR effectors, whereas Pc440 expresses a greater number of RXLRs. This study is the first step toward improving understanding of the molecular basis of differences in the virulence of <i>P. cactorum</i> isolates. Identification of the key effectors is important, as it enables effector-assisted breeding strategies toward crown rot-resistant strawberry cultivars.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" IndexingMethod="Curated" Owner="NLM"><PMID Version="1">30412010</PMID><DateCompleted><Year>2019</Year><Month>08</Month><Day>19</Day></DateCompleted><DateRevised><Year>2019</Year><Month>08</Month><Day>19</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Print">0031-949X</ISSN><JournalIssue CitedMedium="Print"><Volume>109</Volume><Issue>5</Issue><PubDate><Year>2019</Year><Month>May</Month></PubDate></JournalIssue><Title>Phytopathology</Title><ISOAbbreviation>Phytopathology</ISOAbbreviation></Journal><ArticleTitle>Expression of the GAF Sensor, Carbohydrate-Active Enzymes, Elicitins, and RXLRs Differs Markedly Between Two <i>Phytophthora cactorum</i> Isolates.</ArticleTitle><Pagination><MedlinePgn>726-735</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1094/PHYTO-04-18-0136-R</ELocationID><Abstract><AbstractText>The phytopathogen <i>Phytophthora cactorum</i> infects economically important herbaceous and woody plant species. <i>P. cactorum</i> isolates differ in host specificity; for example, strawberry crown rot is often caused by a specialized pathotype. Here we compared the transcriptomes of two <i>P. cactorum</i> isolates that differ in their virulence to garden strawberry (Pc407: high virulence; Pc440: low virulence). De novo transcriptome assembly and clustering of contigs resulted in 19,372 gene clusters. Two days after inoculation of <i>Fragaria vesca</i> roots, 3,995 genes were differently expressed between the <i>P. cactorum</i> isolates. One of the genes that were highly expressed only in Pc407 encodes a GAF sensor protein potentially involved in membrane trafficking processes. Two days after inoculation, elicitins were highly expressed in Pc407 and lipid catabolism appeared to be more active than in Pc440. Of the carbohydrate-active enzymes, those that degrade pectin were often more highly expressed in Pc440, whereas members of glycosyl hydrolase family 1, potentially involved in the metabolism of glycosylated secondary metabolites, were more highly expressed in Pc407 at the time point studied. Differences were also observed among the RXLR effectors: Pc407 appears to rely on a smaller set of key RXLR effectors, whereas Pc440 expresses a greater number of RXLRs. This study is the first step toward improving understanding of the molecular basis of differences in the virulence of <i>P. cactorum</i> isolates. Identification of the key effectors is important, as it enables effector-assisted breeding strategies toward crown rot-resistant strawberry cultivars.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Toljamo</LastName><ForeName>Anna</ForeName><Initials>A</Initials><Identifier Source="ORCID">http://orcid.org/0000-0002-7602-6982</Identifier><AffiliationInfo><Affiliation>Department of Environmental and Biological Sciences, University of Eastern Finland, FI-70211 Kuopio, Finland.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Blande</LastName><ForeName>Daniel</ForeName><Initials>D</Initials><AffiliationInfo><Affiliation>Department of Environmental and Biological Sciences, University of Eastern Finland, FI-70211 Kuopio, Finland.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Munawar</LastName><ForeName>Mustafa</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>Department of Environmental and Biological Sciences, University of Eastern Finland, FI-70211 Kuopio, Finland.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Kärenlampi</LastName><ForeName>Sirpa O</ForeName><Initials>SO</Initials><AffiliationInfo><Affiliation>Department of Environmental and Biological Sciences, University of Eastern Finland, FI-70211 Kuopio, Finland.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Kokko</LastName><ForeName>Harri</ForeName><Initials>H</Initials><AffiliationInfo><Affiliation>Department of Environmental and Biological Sciences, University of Eastern Finland, FI-70211 Kuopio, Finland.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2019</Year><Month>04</Month><Day>05</Day></ArticleDate></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Phytopathology</MedlineTA><NlmUniqueID>9427222</NlmUniqueID><ISSNLinking>0031-949X</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D002241">Carbohydrates</NameOfSubstance></Chemical></ChemicalList><MeshHeadingList><MeshHeading><DescriptorName UI="D002241" MajorTopicYN="N">Carbohydrates</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D031985" MajorTopicYN="N">Fragaria</DescriptorName><QualifierName UI="Q000382" MajorTopicYN="Y">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D050356" MajorTopicYN="N">Lipid Metabolism</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010838" MajorTopicYN="N">Phytophthora</DescriptorName><QualifierName UI="Q000145" MajorTopicYN="Y">classification</QualifierName><QualifierName UI="Q000201" MajorTopicYN="N">enzymology</QualifierName><QualifierName UI="Q000472" MajorTopicYN="N">pathogenicity</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010935" MajorTopicYN="N">Plant Diseases</DescriptorName><QualifierName UI="Q000382" MajorTopicYN="Y">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D064210" MajorTopicYN="N">Secondary Metabolism</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D059467" MajorTopicYN="Y">Transcriptome</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D014774" MajorTopicYN="N">Virulence</DescriptorName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>2018</Year><Month>11</Month><Day>10</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2019</Year><Month>8</Month><Day>20</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2018</Year><Month>11</Month><Day>10</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">30412010</ArticleId><ArticleId IdType="doi">10.1094/PHYTO-04-18-0136-R</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Finlande</li></country></list><tree><country name="Finlande"><noRegion><name sortKey="Toljamo, Anna" sort="Toljamo, Anna" uniqKey="Toljamo A" first="Anna" last="Toljamo">Anna Toljamo</name></noRegion><name sortKey="Blande, Daniel" sort="Blande, Daniel" uniqKey="Blande D" first="Daniel" last="Blande">Daniel Blande</name><name sortKey="K Renlampi, Sirpa O" sort="K Renlampi, Sirpa O" uniqKey="K Renlampi S" first="Sirpa O" last="K Renlampi">Sirpa O. K Renlampi</name><name sortKey="Kokko, Harri" sort="Kokko, Harri" uniqKey="Kokko H" first="Harri" last="Kokko">Harri Kokko</name><name sortKey="Munawar, Mustafa" sort="Munawar, Mustafa" uniqKey="Munawar M" first="Mustafa" last="Munawar">Mustafa Munawar</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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